35mm culture dishes
Luminescence measurement device for real-time reporter assay

AB-2550 Kronos Dio

AB-2550 Kronos Dio is a luminescence measurement device (luminometer) that equips a photomultiplier tube as a detector. This device is suitable to monitor gene expression of culture cells and cultured tissue slice at fixed intervals over a period of several hours to several days using 35mm diameter culture dish as a sample container.
For the long term cultures, it is possible not only to control temperature in the cabinet by the air circulation but also to introduce CO. Additionally, because an optical filter is built in, it is possible to monitor up to three kinds of gene expression using luciferase with different colors of luminescence.

Flow of sample measurement using Kronos Dio
Kronos Dio measures luminescence of the cells in the culture dish.

To measure living cells

In the chamber, the temperature is kept at a constant level and CO2 gas can be introduced. For general cultures of cells, constant temperature conditions of 30 to 40 degrees are essential. AB-2550 Kronos Dio is equipped with a constant temperature device using Peltier device, so it is possible to maintain the temperature in the chamber at a constant level (25 to 45 degrees:room temperature 25 degrees). Moreover, for cells that need to be cultured with CO2 introduced, it is possible to maintain CO2 density at 5% (CO2 sensor and regulator equipped)

There is a turntable in the chamber of Kronos Dio, and eight 35mm diameter culture dishes can be set. There is a detector (condenser lens + photomultiplier tube) under position A in the photo.

 Applicable to long term measurements and multicolor luminescence measurement

Kronos Dio can hold up to eight 35 mm diameter culture dishes in the chamber. The turntable has a structure which no stray light can cause interference between each dish. Because dishes can be exchanged easily by opening the door of the chamber, stimulation with drugs can easily be performed. After the door is closed, temperature rises promptly, so a stable culture condition can be obtained. Luminescence is measured for long time at regular intervals. This is applicable to multicolor assay measurement to obtain luminescence of three colors using one substrate because a color separation mechanism is built-in.

The inner filters of Kronos Dio are switched sequentially and luminescence permeating through the filter is measured by the photomultiplier tube.